Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (Tris-HCl-8%)
Sample
Mouse Cell lysate - other (NIH-3T3 cells_Cytosolic and Nuclear fractions)
Specification
NIH-3T3 cells_Cytosolic and Nuclear fractions
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 76 kDa
Exposure
10 second(s)
Positive control
Cytosolic and Nuclear fractions of NIH-3T3 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 09 2013