Rabbit polyclonal to LYPLA2
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (CGNTMSVPLL TDAATVSGAE RETAAVIFLH GLGDTGHSWA DALSTIRLPH) of Human LYPLA2 (NP_009191).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
May hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has lysophospholipase activity (By similarity). Deacylates GAP43.
Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.
Information by UniProt
- Acyl protein thioesterase 2 antibody
- Acyl protein thioesterase antibody
- Acyl-protein thioesterase 2 antibody
Western blot - Anti-LYPLA2 antibody (ab87231)
Anti-LYPLA2 antibody (ab87231) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
25 kDaObserved band size:
31 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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