LATS1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to LATS1
アプリケーション適用あり: WB, IPmore details
種交差性交差種: Mouse, Human
交差が予測される動物種: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residues 50 and 100 of human LATS1 (SwissProt: O95835).
- Whole cell lysate from HeLa Cells.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）ab70561 was affinity purified using an epitope specific to LATS1 (WARTS) immobilized on solid support.
Our Abpromise guarantee covers the use of ab70561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
機能Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
組織特異性Expressed in all adult tissues examined except for lung and kidney.
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
翻訳後修飾Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
- Information by UniProt
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
All lanes : Anti-LATS1 antibody (ab70561) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 200 kDa, 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Detection of human LATS1 by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70561 at 3 µg/mg of lysate (lane 1).
LATS1 was also immunoprecipitated with an other LATS1 antibody (lanes 2) and with ab70562 (lane 3) using 3 µg/mg lysate.
Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1 was performed using ab70561 at 0.1 µg/ml.
This product has been referenced in:
- Verma A et al. Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction. Oncotarget 9:8068-8080 (2018). Read more (PubMed: 29487715) »
- Li L et al. The inhibition of Hippo/Yap signaling pathway is required for magnesium isoglycyrrhizinate to ameliorate hepatic stellate cell inflammation and activation. Biomed Pharmacother 106:83-91 (2018). Read more (PubMed: 29957470) »