Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic fibriblast)
Loading amount
50 µg
Specification
embryonic fibriblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 3% milk in TBS-Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 110 kDa Non-specific: 40 kDa
Positive control
Another monoclonal antibody against the luminal domain of LAMP-2 detects the same 110 kDa band.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eeva-Liisa Eskelinen
Verified customer
投稿 Dec 11 2007