L-Amino Acid Assay Kit (ab65347)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 40 µM
製品の概要
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製品名
L-Amino Acid Assay Kit -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
<= 40 µM -
全工程の試験時間
0h 30m -
製品の概要
L-Amino Acid Assay Kit ab65347 is used measure the level of L-amino acids in biological samples. There is no requirement for sample pretreatment or purification.
The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ = 570 nm) methods in 96-well plates.
Amino acid assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K639 L-Amino Acid Quantitation Colorimetric/Fluorometric Kit. K639-100 is the same size as the 100 test size of ab65347.
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer II 1 x 25ml L-Amino Acid Enzyme Mix 1 vial L-Amino Acid Standard 1 x 300µl OxiRed Probe 1 x 0.2ml -
研究分野
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関連性
Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all but glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab65347 は 22 報の論文で使用されています。
- Elmelund E et al. Opposing effects of chronic glucagon receptor agonism and antagonism on amino acids, hepatic gene expression, and alpha cells. iScience 25:105296 (2022). PubMed: 36325048
- Hope DCD et al. Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Cell Rep Med 3:100810 (2022). PubMed: 36384093
- Guimarães M et al. Can Metabolite and Hormone Profiles Provide a Rationale for Choosing Between Bariatric Procedures? Obes Surg 31:2174-2179 (2021). PubMed: 33511559
- Olennikov DN et al. Water-Soluble Melanoidin Pigment as a New Antioxidant Component of Fermented Willowherb Leaves (Epilobium angustifolium). Antioxidants (Basel) 10:N/A (2021). PubMed: 34439548
- Modvig IM et al. Amino acids differ in their capacity to stimulate GLP-1 release from the perfused rat small intestine and stimulate secretion by different sensing mechanisms. Am J Physiol Endocrinol Metab 320:E874-E885 (2021). PubMed: 33645250