Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate-Na, 0.05%Tween20, pH6.0., and cycle was on 120C 10 min using autoclave
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 8% · Temperature: 37°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DAKO antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: HitoFine Simple Stain MAX-PO (MULTI)
Additional data
Additional Notes
Coloured with DAB, counterstaining was hematoxylin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 01 2012