Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic islet cells)
Loading amount
50 µg
Specification
pancreatic islet cells
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Positive control
NO
Negative control
No
Additional data
Additional Notes
We used different conditions and repeated multiple times, and still wasn't able to detect any insulin/proinsulin signaling.
Abcam response
Thank you for your review of this untested application. We do value your feedback on the use of this antibody in Western Blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sarah Wang
Verified customer
投稿 May 15 2013