製品の概要

  • 製品名
    Anti-Influenza A Virus Nucleoprotein antibody [C43]
    Influenza A Virus Nucleoprotein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
  • 由来種
    Mouse
  • 特異性

    Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).

    No cross reactivity with influenza B viruses.

  • アプリケーション
    適用あり: Flow Cyt, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Influenza A
  • 免疫原

    Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
    Database link: P69291

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • 精製度
    Proprietary Purification
  • 特記事項(精製)
    Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE
  • ポリ/モノ
    モノクローナル
  • クローン名
    C43
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab128193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 24067955

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB 1/300 - 1/1000.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200.

ターゲット情報

  • 関連性
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • 細胞内局在
    Host cell nucleus
  • 参照データベース
    • 別名
      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    画像

    • Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
    • Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

      Secondary
      Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
    • Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
    • All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution

      Lanes 1 & 6 & 11 : MDCK cells mock infected
      Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
      Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
      Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
      Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
      Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
      Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
      Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
      Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
      Lane 12 : MDCK cells infected H5N1
      (A/duck/HK/342/78)collected 3 hrs post-infection
      Lane 13 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 9 hrs post-infection
      Lane 14 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 24 hrs post-infection
      Lane 15 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 48 hrs post-infection


      Tubulin was used as loading control.

    参考文献

    This product has been referenced in:
    • Kupke SY  et al. A Novel Type of Influenza A Virus-Derived Defective Interfering Particle with Nucleotide Substitutions in Its Genome. J Virol 93:N/A (2019). Read more (PubMed: 30463972) »
    • Welch D  et al. Far-UVC light: A new tool to control the spread of airborne-mediated microbial diseases. Sci Rep 8:2752 (2018). ICC/IF . Read more (PubMed: 29426899) »
    See all 6 Publications for this product

    レビューと Q&A

    1-5 of 5 Abreviews or Q&A

    Answer

    The immunogen of this antibody is human Influenza A Virus (H2N2) Okada strain.

    Unfortunately, we have not determined the epitope recognized by this antibody.

    Read More

    Question
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1230375.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for your response to my further questions. It is very much appreciated.

    When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make sure that the experiments are nicely controlled. Without these details and the exact strains of the virus, it is hard to troubleshoot and to provide sufficient technical support.

    We have not received any similar complaint about ab128193, thus not aware of any QC issues. Currently, we have the same batch in stock your customer has tried. I could offer you a new vial of ab128193 or one vial of any alternative antibodies against the same target protein (Influenza A Virus Nucleoprotein).

    We have the following antibodies which are suitable for Western blot application:

    - https://www.abcam.com/ab43821: Anti-Influenza A Virus Nucleoprotein antibody [9G8]

    - https://www.abcam.com/ab66191: Anti-Influenza A Virus Nucleoprotein antibody [B247M]

    - https://www.abcam.com/ab81126: Anti-Influenza A Virus Nucleoprotein antibody [15]

    Please could you discuss it with your customer and let me know how he/she would like to proceed.

    I look forward to hearing from you soon.

    Read More

    Answer

    Thank you for contacting us.

    The immunogen of ab128193 is whole virus of Human Influenza AVirus (H2N2) OKada strain. We, however, have not mapped the epitope of this antibody.

    I am sorry for not being able to provide you any useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    I am sorry if ab128193 did not work as you expected.

    H7N3 subtype of avian Influenza A virus has not been tested with this antibody. I would recommend you to test IF with the virus-infected cells or ELISA with inactivated purified antigen of N7H3 to examine the reactivity of C43 antibody with N7H3 subtype.

    In regards to ab43821, it should recognize H7N3 influenza; however, it was not tested on this viral strain.

    You may have received the quotation in a separate email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

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