Anti-ITIH1/SHAP 抗体 (ab110822)
Key features and details
- Rabbit polyclonal to ITIH1/SHAP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ITIH1/SHAP antibody
ITIH1/SHAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ITIH1/SHAP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee -
免疫原
Synthetic peptide corresponding to ITIH1/SHAP.
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ポジティブ・コントロール
- Recombinant Human ITIH1/SHAP protein (ab114721) can be used as a positive control in WB. Human OA chondrocyte tissue, mouse OA periosteum tissue ICC/IF: HepG2 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 2 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 2 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells. -
配列類似性
Belongs to the ITIH family.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
翻訳後修飾
Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.
The S-linked glycan is composed of two 6-carbon sugars, possibly Glc or Gal. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3697 Human
- Entrez Gene: 16424 Mouse
- Omim: 147270 Human
- SwissProt: P19827 Human
- SwissProt: Q61702 Mouse
- Unigene: 420257 Human
- Unigene: 3227 Mouse
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別名
- H1P antibody
- IATIH antibody
- IGHEP1 antibody
see all
画像
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ab110822 staining ITIH1/SHAP in Human OA chondrocyte tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH1/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
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Immunofluorescence analysis of ITIH1 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab110822 at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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ab110822 staining ITIH1/SHAP in Human OA chondrocyte tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH1/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
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ab110822 staining ITIH1/SHAP in Mouse OA periosteum tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH1/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110822 は論文での使用が確認できていません。