製品名Anti-IRAKM antibody [Y278]
IRAKM 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [Y278] to IRAKM
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC or IHC
Synthetic peptide within Human IRAKM aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Y616
- A431 cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab32394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
機能Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
組織特異性Expressed predominantly in peripheral blood lymphocytes.
関連疾患Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
- Information by UniProt
- ASRT5 antibody
- IL 1 receptor associated kinase M antibody
- IL-1 receptor-associated kinase M antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa
ab32394 has not yet been referenced specifically in any publications.