Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW macrophages cell line)
Loading amount
50 µg
Specification
RAW macrophages cell line
Treatment
LPS and peptidoglycan
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 5% milk in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: sc-2030
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ecl+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: no kDa Non-specific: ~100KD kDa
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Varkha Agrawal
Verified customer
投稿 Sep 11 2012