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Synthetic peptide corresponding to Human IL23 (C terminal). 14 amino acid peptide located within the last 50 amino acids of IL23.
Our Abpromise guarantee covers the use of ab45420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).|
Immunohistochemistry of IL-23 in human pancreas tissue with ab45420 at 5 µg/mL.
Ab45420 staining IL23 in Mouse bone marrow derived macrophages by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody at 1/200 dilution in 5% goat serum in PBS for 1 hour at 20°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.
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