Anti-IGF2 抗体 (ab9574)
Key features and details
- Rabbit polyclonal to IGF2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IGF2 antibody
IGF2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IGF2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IGF2. Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
Database link: P01344 -
特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
保存方法
Shipped at 4°C. Store at -20ºC. -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9574の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
特記事項 |
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WB
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. |
ターゲット情報
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機能
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
関連疾患
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
配列類似性
Belongs to the insulin family. -
翻訳後修飾
O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
画像
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ab9574 detecting IGF2 using sandwich ELISA
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Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
Lane 1: Marker
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (105)
ab9574 は 105 報の論文で使用されています。
- Sepúlveda D et al. Insulin-like growth factor 2 and autophagy gene expression alteration arise as potential biomarkers in Parkinson's disease. Sci Rep 12:2038 (2022). PubMed: 35132125
- Yu Y et al. Circ_0026579 alleviates LPS-induced WI-38 cells inflammation injury in infantile pneumonia. Innate Immun 28:37-48 (2022). PubMed: 35142578
- Yuan Z et al. Magnesium isoglycyrrhizinate suppresses bladder cancer progression by modulating the miR-26b/Nox4 axis. Bioengineered 13:7986-7999 (2022). PubMed: 35293283
- Lehman NL et al. Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes. Nat Commun 13:2083 (2022). PubMed: 35440587
- Catalano R et al. The cytoskeleton actin binding protein filamin A impairs both IGF2 mitogenic effects and the efficacy of IGF1R inhibitors in adrenocortical cancer cells. Cancer Lett 497:77-88 (2021). PubMed: 33075426