Anti-IGF1 抗体 (ab9572)
Key features and details
- Rabbit polyclonal to IGF1
- Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IGF1 antibody
IGF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IGF1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Database link: P05019 -
ポジティブ・コントロール
- IHC-P: Human pancreas and liver tissues.
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9572の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (2) |
Use at an assay dependent concentration.
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IHC-FoFr | (1) |
Use at an assay dependent concentration. PubMed: 28754163
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
|
IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.
|
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28754163 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
ターゲット情報
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機能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
関連疾患
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Omim: 147440 Human
- SwissProt: P01343 Human
- SwissProt: P05019 Human
- SwissProt: P05017 Mouse
- Unigene: 160562 Human
- Unigene: 268521 Mouse
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製品の状態
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
別名
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (104)
ab9572 は 104 報の論文で使用されています。
- Kamaludin Z et al. Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women. Pathophysiology 29:9-23 (2022). PubMed: 35366286
- Ballester-Rosado CJ et al. A Role for Insulin-like Growth Factor 1 in the Generation of Epileptic Spasms in a murine model. Ann Neurol 92:45-60 (2022). PubMed: 35467038
- Wang X et al. Therapeutic Effects of Retinoic Acid in Lipopolysaccharide-Induced Cardiac Dysfunction: Network Pharmacology and Experimental Validation. J Inflamm Res 15:4963-4979 (2022). PubMed: 36105385
- Park JY et al. The Potential of Transforming Growth Factor-beta Inhibitor and Vascular Endothelial Growth Factor Inhibitor as Therapeutic Agents for Uterine Leiomyoma. Int J Med Sci 19:1779-1786 (2022). PubMed: 36313223
- Takata S et al. LIF-IGF Axis Contributes to the Proliferation of Neural Progenitor Cells in Developing Rat Cerebrum. Int J Mol Sci 23:N/A (2022). PubMed: 36361987