Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)


  • 製品名
    Hydroxymethylated DNA Quantification Kit (Fluorometric)
    hydroxymethylated DNA キット 製品一覧
  • サンプルの種類
    Saliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
  • アッセイタイプ
  • 検出感度
    >= 10 pg/well
  • 全工程の試験時間
    3h 20m
  • 種交差性
    交差種: Other species, Mammals
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

    Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.

    It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.


  • 保存方法
    Please refer to protocols.
  • 内容 48 tests 96 tests
    10X Wash Buffer 1 x 14ml 1 x 28ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Binding Solution 1 x 5ml 1 x 10ml
    Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl
    Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl
    Enhancer Solution 1 x 8µl 1 x 16µl
    Fluoro Developer 1 x 8µl 1 x 16µl
    Fluoro Dilutor 1 x 4ml 1 x 8ml
    Fluoro Enhancer 1 x 8µl 1 x 16µl
    Negative Control I, 20 µg/mL 1 x 10µl 1 x 20µl
    Negative Control II, 20 µg/mL 1 x 10µl 1 x 20µl
    Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl
  • 研究分野


  • Demonstration of high sensitivity and specificity of 5-hydroxymethylcytosine detection achieved using ab117131. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117131.



This product has been referenced in:
  • Pinzón-Cortés JA  et al. Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation. Endocr Connect 6:708-725 (2017). Read more (PubMed: 28993426) »

See 1 Publication for this product

レビューと Q&A

High variability

Average Good 4/5 (Ease of Use)
The kit is relatively easy to use and mercifully has less steps than the methylation assay kit ab117129.
There was high variability between my duplicate samples even though I followed the instructions very closely.

Abcam user community

Verified customer

投稿 Aug 20 2018

Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

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Thank you for contacting Abcam regarding our EpiSeeker Hydroxymethylated DNA Quantification kits.

The main difference between the colorimetric and fluorometric kits is the method of detection - this will require either a microplate read...

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I would like to inform you that we are still investigating the problem you experienced with the kits. The lab has already provided the following information: "The reading can be carried out at 530/590 nm +/- 10 generally. Thus, emission at 59...

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