Human Soluble TNF Receptor II ELISA Kit (ab100643)


  • 製品名
    Human Soluble TNF Receptor II ELISA Kit
    Soluble TNF Receptor II キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    100 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 102.65 91% - 112%
    Serum 104.92 95% - 115%
    Plasma 105.49 94% - 118%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Soluble TNF Receptor II ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Soluble TNF Receptor II in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Soluble TNF Receptor II coated on a 96-well plate. Standards and samples are pipetted into the wells and Soluble TNF Receptor II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Soluble TNF Receptor II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Soluble TNF Receptor II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Soluble TNF Receptor II (lyophilized) 2 vials
    Recombinant Human Soluble TNF Receptor II Standard (lyophilized) 2 vials
    Soluble TNF Receptor II Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    TNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain. Soluble TNFRII (Soluble TNF Receptor II) is a 268 amino acid isoform containing the extracellular ligand-binding portion of TNFRII. It binds to TNF alpha and is capable of inhibiting TNF alpha induced activities by acting as a decoy receptor.
  • 別名
    • CD120b
    • Etanercept
    • p75
    • p80 TNF alpha receptor
    • TBP2
    • TBPII
    • TNFR2
    • TNFRII
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily member 1B
    • Tumor necrosis factor receptor superfamily member 1b, membrane form
    • Tumor necrosis factor receptor type II
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100643

  • Representative standard curve using ab100643



ab100643 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your recent message.

I can confirm that this kit (Soluble human TNF Receptor II) is suitable for detecting Etanarcept which is an alternative name for TBPII. We have not specifically tested with these drugs. As long as your samp...

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Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample prep...

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