Human Plasminogen ELISA Kit (PLG) (ab108893)
Key features and details
- Sensitivity: 0.26 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Plasminogen ELISA Kit (PLG)
Plasminogen キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.26 ng/ml -
検出範囲
0.625 ng/ml - 40 ng/ml -
添加回収試験
= 101 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Plasminogen ELISA kit (PLG) is designed for the quantitative measurement of Plasminogen (PLG) concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid, cell culture, and cell lysate samples.
A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.
Get higher sensitivity in only 90 minutes with Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Plasminogen Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Plasminogen Microplate (12 x 8 well strips) 1 unit Plasminogen Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
組織特異性
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
関連疾患
Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
ドメイン
Kringle domains mediate interaction with CSPG4. -
翻訳後修飾
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
細胞内局在
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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別名
- Plasmin
- Plasmin heavy chain A
- Plasmin light chain B
see all -
参照データベース
- Entrez Gene: 5340 Human
- Omim: 173350 Human
- SwissProt: P00747 Human
- Unigene: 143436 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108893 は 3 報の論文で使用されています。
- Cabrera-Garcia D et al. Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients. Int J Hematol 116:937-946 (2022). PubMed: 35994163
- Zitur LJ et al. Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression. Transplantation 103:1821-1833 (2019). PubMed: 30964836
- Banville N et al. A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM). PLoS One 9:e105365 (2014). Sandwich ELISA ; Human . PubMed: 25133674