Human PMN Elastase ELISA Kit (ab119553)
Key features and details
- Sensitivity: 1.98 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PMN Elastase ELISA Kit
Neutrophil Elastase キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 5.6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.98 pg/ml -
検出範囲
0.16 ng/ml - 10 ng/ml -
添加回収試験
104 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Plasma 104 96% - 110% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests Adhesive Films 2 units Control high 1 vial Control low 1 vial HRP-Conjugate anti-alpha1-PI polyclonal antibody 1 x 16ml Human PMN Elastase - alpha1-PI complex Standard, lyophilized, (10 ng/mL upon reconstitution) 1 vial Microplate coated with polyclonal antibody to Human PMN Elastase (12 x 8 wells) 1 unit Sample Diluent 1 x 50ml Stop Solution 1 x 7ml TMB Substrate Solution 1 x 22ml Wash Buffer Concentrate (10x) 1 x 50ml -
研究分野
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機能
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
組織特異性
Bone marrow cells. -
関連疾患
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
配列類似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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別名
- Bone marrow serine protease
- ELA2
- ELANE
see all -
参照データベース
- Entrez Gene: 1991 Human
- Omim: 130130 Human
- SwissProt: P08246 Human
- Unigene: 99863 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab119553 は 28 報の論文で使用されています。
- Louis Sam Titus ASC et al. Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens. Mol Cell Proteomics 22:100487 (2023). PubMed: 36549591
- Lodge KM et al. Hypoxia Increases the Potential for Neutrophil-mediated Endothelial Damage in Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med 205:903-916 (2022). PubMed: 35044899
- Cedervall J et al. Neutrophil extracellular traps promote cancer-associated inflammation and myocardial stress. Oncoimmunology 11:2049487 (2022). PubMed: 35309730
- D'Amato M et al. Investigating the Link between Alpha-1 Antitrypsin and Human Neutrophil Elastase in Bronchoalveolar Lavage Fluid of COVID-19 Patients. Curr Issues Mol Biol 44:2122-2138 (2022). PubMed: 35678672
- Fungmongkonsatean T et al. Hemocompatibility Evaluation of Thai Bombyx mori Silk Fibroin and Its Improvement with Low Molecular Weight Heparin Immobilization. Polymers (Basel) 14:N/A (2022). PubMed: 35890719