Blocking peptide for WB

Western blot
We have used whole kidney lysate form normoglycemic mice or STZ induced diabetic mice after 16 weeks from injection.

Application: Western blot
Sample: whole Kidney lysate
Gel Running Conditions: Reduced Denaturing
Loading amount: 30 µg

Treatment: Five consecutive intraperitoneal Strreptozotocin injections (Cayman Chemical 50 mg/kg per day), Samples from kidneys after 16 weeks from last injection.
Specification: Mouse Kidney
Blocking step: Milk as blocking agent for 1 hour, Temperature: 25°C

First antibody (ab95256) with/ without Blocking peptide (ab105630), ratio 1:10-
Incubation time 16 hour(s) , Temperature: 4°C
Dilution 1/1000

Secondary antibody
Dilution 1/10000
Name ab97085
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection method ECL+
Exposure 2 minute(s) and 0 second(s)

lanes 1-3, 7-9 are from WT normoglycemic mice
lanes 4-6, 10-12 are from STZ induced diabetic mice

lanes 1-6 with ab95256
lanes 7-12 with ab95256+ ab105630

STZ- Streptozotocine
bp- Blocking peptide

Shiran Udi

Verified customer

投稿 Jul 03 2017