製品の概要

  • 製品名
    Human G-CSF ELISA Kit
    G-CSF キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 1 pg/ml
  • 検出範囲
    0.69 pg/ml - 500 pg/ml
  • 添加回収試験

    > 75 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 76.56 68% - 90%
    Serum 83.45 72% - 94%
    Plasma 74.76 67% - 87%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.


     

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    260X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human G-CSF (lyophilized) 2 vials
    G-CSF Microplate (12 x 8 wells) 1 unit
    Recombinant Human G-CSF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
  • 別名
    • C17orf33
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3OS
    • Csfg
    • Filgrastim
    • GCSA
    • GCSF
    • Granulocyte colony stimulating fact
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • MGI IG
    • OTTMUSP00000006785
    • Pluripoietin
    • RP23-395E10.4
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).

  • Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).

  • Representative standard curve using ab100524

  • G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).

  • Representative standard curve using ab100524

プロトコール

参考文献

ab100524 has not yet been referenced specifically in any publications.

レビューと Q&A

1-8 of 8 Abreviews or Q&A

Question
Answer

We have not tested the GCSF ELISA kit with Cynomolgus monkey serum. However, when looking at the human GCSF sequence our kit recognizes and testing the cross-reactivity to the Cynomolgus sequence, there are a number of predicted matches, including granulocyte colony-stimulating factor (isoforms X1-4), with which it has 94% homology. Thus, it is likely that the kit will work with Cynomologus serum. Especially since we have a 90 day 100% risk-free policy for ELISAs, there is no risk in this case to try it.

Please keep in mind though that there are a number of other predicted matches (other than GCSF) with varying homology that it may cross-react with and there is not a 100% sequence match. So you will have to consider this when interpreting any results.

In case you are interested in buying and trying this product then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in your next order in-exchange for review of this product. Please contact me with the order number for Abtrial code once the purchase is completed.


More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

This Abtrial offer was created to give scientists the opportunity to try products in an untested species or application, without the financial risk of failure.

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Question
Answer



The standard recombinant protein of ab100524 was expressed in E.coli.

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Answer

The standard we use for this kit (ab100524) is recombinant human G-CSF: 18.7 kDa protein; 174 amino acid residues. We have not tested this kit with a met-GCSF, but the following amino acid sequence of the recombinant standard used should help the customer determine whether or not the antibody pairs used will recognize the met-GCSF as well:

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

If your customer has the sequence of the methionyl GCSF, we could compare the sequences to see whether this kit is likely to work on methionyl GCSF.

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Answer

Thank you for contacting us.

Serum can be stored at -80C for a month, this will be having minimal affect on the assay.

The following publication will help understanding the affect of temperature on different serum protein.

http://www.annclinlabsci.org/content/40/1/61.full.pdf+html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your reply.

The strips come with a rack where the single strips can be inserted.

This rack will fit all standard plate readers.

I am sorry, I cannot check if this is also true for your plate reader. Please ask the supplier of the instrument.

I hope this information is helpful and wish you good luck with experiments.

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Answer

Thank you for your inquiry.

I can confirm that with ab100524 it is not necessary to use the whole plate at one time.

The plate comes in convenient 12 strips x 8 wells.

Please follow the instruction of the protocol when storing opened plate and solutions:

"

ab100524 may be stored for up to 6 months at 2 to 8°C from the

date of shipment. Standard (recombinant protein) should be stored

at -20°C or -80°C (recommended at –80°C) after reconstitution.

Opened Microplate Wells or reagents may be store for up to 1 month

at 2 to 8°C. Return unused wells to the pouch containing desiccant

pack, reseal along entire edge. Note: the kit can be used within one

year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw

cycles."

I hope this information is helpful and wish you good luck with your experiments.

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Answer

Thank you for contacting Abcam.

We still have not tested whether the kit can be used to quantitate PEGfilgrastim, therefore the original response, "to spike in pegylated G-CSF and see" is still the only option for answering this question.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for your inquiry.  That is a good question.  I reviewed our testing records on ab100524 but unfortunately I was not able to find any data about pegylated G-CSF.  Therefore we don’t know if this kit will also detect the pegylated form.  The only way to know would be to spike in pegylated G-CSF and see.   I wish I could be of more help, but please let us know if you have any other questions.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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