Rabbit polyclonal to Hormone sensitive lipase
Synthetic peptide corresponding to a region within C terminal amino acids 828-858 of Human HSL/LIPE (Q05469), conjugated to KLH.
HL-60 cell lysate; Human testis tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 117 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Glycerolipid metabolism; triacylglycerol degradation.
Belongs to the 'GDXG' lipolytic enzyme family.
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
Information by UniProt
Hormone sensitive lipase antibody
Hormone sensitive lipase testicular isoform antibody
Hormone-sensitive lipase antibody
Western blot - Anti-Hormone sensitive lipase antibody (ab103281)
Anti-Hormone sensitive lipase antibody (ab103281) at 1/50 dilution + HL-60 cell lysate at 35 µg
Predicted band size: 117 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hormone sensitive lipase antibody (ab103281)
Staining of HSL/LIPE in formalin fixed and paraffin embedded Human testis tissue labelled with ab103281 at a 1/50 dilutuion, which was peroxidase conjugated to the secondary antibody followed by DAB staining.
has not yet been referenced specifically in any publications.
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