Application
Western blot
Sample
Human Cell lysate - whole cell (Primary cells)
Loading amount
20 µg
Specification
Primary cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: NA9340V ECL anti-rabbit HRP (lot:381022)dil in TBS
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ecl
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: ~12 kDa Non-specific: various kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 02 2011