Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
Specification
Fruit fly embryo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA and 0.1% Tween 20 in TBS buffer
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 15 kDa Non-specific: 82 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 21 2014