Application
Western blot
Sample
Human Cell lysate - whole cell (esophageal tumor cells)
Gel Running Conditions
Non-reduced Denaturing (12%SDS)
Loading amount
80 µg
Specification
esophageal tumor cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 5%milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: fluorescence conjugated secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Detection
Detection method
Odyssey
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Qingyuan Yang
Verified customer
投稿 Jan 04 2016