Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (16HBE14o- cells and Caco2 cells)
Specification
16HBE14o- cells and Caco2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk-TBST buffer
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/3000
Detection
Detection method
ECL plus
Bands
Specific: 43 kDa
Exposure
30 second(s)
Additional data
Additional Notes
I also tried longer over-exposure time (10 min). No band around 37kDa is observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 12 2013