Application
Western blot
Sample
Human Cell lysate - whole cell (THP1 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
THP1 Cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL, LiCor C-DIGIT
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 28 kDa Non-specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 27 2018