Application
Western blot
Sample
Human Cell lysate - whole cell (uveal melanoma)
Loading amount
40 µg
Specification
uveal melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
10 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Sigma anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 43kDa kDa Non-specific: approx 27kDa kDa
Positive control
PBMC cell lysate
Negative control
No primary Antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Luminita Paraoan
Verified customer
投稿 Aug 24 2006