Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
none
Permeabilization
Yes - Methanol + 1%H2O2
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Diluent: 4% serum + 1% BSA + 0.01% azide
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated Anti-Mouse IgG (H+L)
Host species: Horse
Clonality: Monoclonal
Conjugation: Biotin
Host species: Horse
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/100
Additional data
Additional Notes
There is loss of antigen with a 10 minutes fixation (4% PFA) prior permeabilisation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Pascal Durrenberger
Verified customer
投稿 Nov 17 2010