Application
Western blot
Sample
Mouse Cell lysate - other (C2C12, MEF)
Loading amount
25 µg
Specification
C2C12, MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: secondary rabbit from invitrogen
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 16, 25 kDa
Additional data
Additional Notes
From left to right: lane1: HepG2, lane2: 293T, lane3: MEF, lane4: C2C12, marker
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked in your hands. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 23 2012