製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. (see Abreview)
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab10829 as Capture.

ターゲット情報

  • 機能

    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 関連疾患

    Deafness, autosomal recessive, 39
  • 配列類似性

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 参照データベース

  • 別名

    • DFNB39 antibody
    • F TCF antibody
    • Fibroblast derived tumor cytotoxic factor antibody
    • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
    • Hepatocyte growth factor antibody
    • Hepatocyte growth factor beta chain antibody
    • Hepatocyte growth factor precursor antibody
    • Hepatopoietin A antibody
    • Hepatopoietin-A antibody
    • Hgf antibody
    • HGF_HUMAN antibody
    • HGFB antibody
    • HPTA antibody
    • Lung fibroblast derived mitogen antibody
    • OTTHUMP00000161349 antibody
    • OTTHUMP00000206710 antibody
    • OTTHUMP00000206711 antibody
    • OTTHUMP00000206712 antibody
    • OTTHUMP00000206713 antibody
    • OTTHUMP00000206730 antibody
    • Scatter factor antibody
    • SF antibody
    see all

画像

  • Standard Curve for HGF (Analyte: ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.
  • Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa


    Exposure time: 1 minute

参考文献

This product has been referenced in:

  • Wen Q  et al. 8-bromo-7-methoxychrysin suppress stemness of SMMC-7721 cells induced by co-culture of liver cancer stem-like cells with hepatic stellate cells. BMC Cancer 19:224 (2019). Read more (PubMed: 30866863) »
  • Kogata Y  et al. Foretinib (GSK1363089) induces p53-dependent apoptosis in endometrial cancer. Oncotarget 9:22769-22784 (2018). Read more (PubMed: 29854314) »
See all 5 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil,cerebellum,fetal liver,normal breast,fetal)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Permeabilization
Yes - 0.05%Tween20 in PBS
Specification
Tonsil,cerebellum,fetal liver,normal breast,fetal
Blocking step
sea block (fisher#37527) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Apr 28 2017

Question
Answer

Thank you for contacting Abcam.

The antibody was generated against the active recombinant protein of HGF, so it should also recognize the inactive precursor form. However we do not have the possibility to check it.

Also, there is a 90% level of homology between the human sequence and mouse, therefore there is strong chance that the antibody will work in this species. As ab24865 has not been tested in mouse samples, you can participate in our AbTrial program. Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human mesenchymal stem cells transduced with Adv-H)
Specification
Human mesenchymal stem cells transduced with Adv-H
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Mr. Hao Wu

Verified customer

投稿 Dec 03 2010

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