Anti-HEXIM1 抗体 (ab58573)
Key features and details
- Goat polyclonal to HEXIM1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HEXIM1 antibody
HEXIM1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to HEXIM1 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow -
免疫原
Synthetic peptide corresponding to Human HEXIM1 aa 346-359 (C terminal).
Sequence:C-HRQQERAPLSKFGD
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ポジティブ・コントロール
- WB: Human and mouse heart tissue lysates. ICC/IF: HeLa and A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. -
組織特異性
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. -
配列類似性
Belongs to the HEXIM family. -
ドメイン
The coiled-coil domain mediates oligomerization. -
細胞内局在
Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 10614 Human
- Entrez Gene: 192231 Mouse
- Entrez Gene: 498008 Rat
- Omim: 607328 Human
- SwissProt: O94992 Human
- SwissProt: Q8R409 Mouse
- SwissProt: Q5M9G1 Rat
- Unigene: 15299 Human
see all -
別名
- Cardiac lineage protein 1 antibody
- CLP 1 antibody
- CLP1 antibody
see all
画像
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All lanes : Anti-HEXIM1 antibody (ab58573) at 0.1 µg/ml
Lane 1 : Human heart tissue lysate in RIPA buffer
Lane 2 : Mouse heart tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDaIncubated with the primary antibody for 1 hour.
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
データシートおよび資料
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Datasheet download
参考文献 (0)
ab58573 は論文での使用が確認できていません。