Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (HA-tag/Myc overexpr. MDA-MB-231 cells, inj.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
HA-tag/Myc overexpr. MDA-MB-231 cells, inj.
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Rabbit anti-Mouse IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
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投稿 May 23 2017