Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

Pré-adsorption est une étape supplémentaire pour augmenter la spécificité d'un anticorps. Je vous envoie un lien pour notre site internet qui explique ce terme en plus de détailes.

https://www.abcam.com/index.html?pageconfig=resource&rid=12956

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Please have a look at the troubleshooting guide given on our website;

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I am sorry I am unable to get the specific protocol for chicken tissues. My colleague from laboratory has suggested that any general IHC-P protocol would work and the staining can be improved with optimization.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply.

While I did not receive the image which you have referenced I would like to offer so help regarding your questions. Generally specific details regarding secondary antibody incubation and concentration as will need to be determined empirically in the lab. These may vary considerable depending upon the secondary antibody used. This is the same for exposure times. We do offer a number of guidelines for information on our datasheet however. Please remember that these are suggested starting points and you will likely need to adjust these to find the optimal ranges.

The secondary antibody AB97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 20 minute exposure time. This product may be found at this location:

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-pre-adsorbed-ab97080.html

The secondary antibody AB65484 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 150 second exposure time. Unfortunately this product is no longer available.

A third experiment is shown on the datasheet; however we do not have specific information as to the product number of the secondary antibody used.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for contacting us.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this.

As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. Once you have received any products, we recommend toplease follow the long-term storage instructions on the datasheet as soon as possible. This will help to ensure that the product remains stable and that we can continue to provide our guarantee.

In this case, considering our findings described above, I would suggest that the antibody should not be significantly affected from being at room temperature for 3 days. We recently redesigned our packing. A decision was taken to label these with 'Temperature sensitive - store according to datasheet instructions' in order to cover all the different products we ship at different temperatures.This instruction should prompt the recipient to check the datasheet, or check with the end user, as soon as possible.

However, I do understand your concerns, and we encourage feedback from customers and take this seriously. I have therefore forwarded your comments on to my colleagues for their reference.

I would like to reassure you that our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found at:

https://www.abcam.com/index.html?pageconfig=contactus

I hope this information helps, but please don't hesitate to contact me for additional information or assistance.

Thank you for your email.
I'm glad to see that the antibody worked.
Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your email.
I'd suggest starting with the recommended dilutions for each antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your email.
The free of charge order was shipped today for delivery tomorrow.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

You are very welcome. I keep my fingers crossed for you and wish you good luck.
Yes, definitely, please keep me informed. I look forward to hear back from you - hopefully with positive results.
Have a nice weekend.