Application
Western blot
Whole Cell lysate - human ES cells.
Loading amount: 10 µg.
Gel Running Conditions: Reduced Denaturing (10%).
Blocking step: 5%BSA/1%OvaAlb/PBS as blocking agent for 3 hours at room temperature.
Primary antibody: Nanog diluted 1:1000 in 1%BSA/0.2%OvaAlb/PBS 13hours at 4°C.
Secondary antibody: ab205719 - Goat Anti-Mouse IgG H&L (HRP) diluted 1:5000 in TBST.
ECL prime: 4 second exposure.
Predicted size: ~45kDa.
Loading amount: 10 µg.
Gel Running Conditions: Reduced Denaturing (10%).
Blocking step: 5%BSA/1%OvaAlb/PBS as blocking agent for 3 hours at room temperature.
Primary antibody: Nanog diluted 1:1000 in 1%BSA/0.2%OvaAlb/PBS 13hours at 4°C.
Secondary antibody: ab205719 - Goat Anti-Mouse IgG H&L (HRP) diluted 1:5000 in TBST.
ECL prime: 4 second exposure.
Predicted size: ~45kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 12 2016