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  1. Link

    goat-mouse-igg-hl-hrp-ab205719.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (614)

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Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Key features and details

  • Goat Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, IP, ELISA, IHC-P

Conjugates logo Related conjugates and formulations

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関連製品

製品の概要

  • 製品名

    Goat Anti-Mouse IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • 由来種

    Goat
  • ターゲット生物種

    Mouse
  • 特異性

    The antibody used for conjugation reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182017): Chicken IgY, less than 2%. Human IgG, less than 6%. Rabbit IgG, less than 7%. Rat IgG, less than 47%.
  • アプリケーション

    適用あり: WB, IP, ELISA, IHC-Pmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

関連製品

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    • Goat Anti-Mouse IgG H&L (ab182017)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Normal Goat Serum (ab7481)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab205719の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/2000 - 1/20000.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/2000 - 1/20000.
特記事項
WB
1/2000 - 1/20000.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/2000 - 1/20000.

画像

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab205719) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab205719, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    IHC image of alpha tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab7291 at 1/1000 dilution. An HRP-conjugated secondary (Ab205719, 1/10000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ab205719 (Left Image) at 1/5000 and a competitor secondary (Right Image) at 1/5000. Notice the decreased signal of the competitor product.

    Performed under reducing conditions.

    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab205719 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    IHC image of histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab31830 at 1/1000 dilution. An HRP-conjugated secondary (Ab205719, 1/10000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    All lanes : No Primary Antibody

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ab205719 (Left Image) 1/2000 and a competitor secondary (Right Image) 1/2000. Notice the increased background of the competitor product.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with ab205719 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.

  • Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab205719) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8226 overnight at 4°C. Antibody binding was detected using ab205719, and visualised using ECL development solution ab133406.

  • ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    This data was developed using the unconjugated antibody (ab182017).

  • ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    ELISA - Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182017).

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (614)

ab205719 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab205719 は 614 報の論文で使用されています。

  • Yu C  et al. CircRNA TGFBR2/MiR-25-3p/TWIST1 axis regulates osteoblast differentiation of human aortic valve interstitial cells. J Bone Miner Metab 39:360-371 (2021). PubMed: 33070258
  • Liu BQ  et al. Fibrinogen-like protein 2 promotes the accumulation of myeloid-derived suppressor cells in the hepatocellular carcinoma tumor microenvironment. Oncol Lett 21:47 (2021). PubMed: 33281958
  • Peng T  et al. miR-125/CDK2 axis in cochlear progenitor cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33300064
  • Wang X  et al. Penehyclidine hydrochloride alleviates lipopolysaccharide-induced acute respiratory distress syndrome in cells via regulating autophagy-related pathway. Mol Med Rep 23:N/A (2021). PubMed: 33300058
  • Qu H  et al. HIF-3a affects preeclampsia development by regulating EVT growth via activation of the Flt-1/JAK/STAT signaling pathway in hypoxia. Mol Med Rep 23:N/A (2021). PubMed: 33215219
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

abreview for ab205719 - Goat Anti-Mouse IgG H&L (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
We did the following experiments to test Goat Anti-Mouse IgG H&L (HRP) as a secondary antibody.

Rat Tissue lysate - whole (cardiac muscle)
Loading amount: 20 µg
Specification: cardiac muscle

Blocking step: Milk as blocking agent for 1 hour (Concentration: 5 % · Temperature: 22°C)
Primary antibody: Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
Dilution: 1/5000, Incubation time: 18 hours Temperature: 4°C.

Secondary antibody: Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Dilution: 1/5000, Incubation time: 1 hour Temperature: 22°C.

Detection method: ECL
Exposure: 2 minutes
Bands: Specific: (50 kDa)

Abcam user community

Verified customer

投稿 Jan 27 2022

Goat Anti-Mouse IgG with Oxphos rodent antibody (ab110413)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
- 20ug of bovine liver lysates were loaded on to 4-15% gradient gel
- Blocked for an hour in 5% skim milk
- 1:4000 primary antibody used (ab110413). Incubation O/N @4C
- 1:20000 secondary antibody used (ab205719). Incubation 1 Hour room temperature.

Julie Kim

Verified customer

投稿 Apr 21 2017

Nanog Western Blot

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Whole Cell lysate - human ES cells.
Loading amount: 10 µg.
Gel Running Conditions: Reduced Denaturing (10%).
Blocking step: 5%BSA/1%OvaAlb/PBS as blocking agent for 3 hours at room temperature.
Primary antibody: Nanog diluted 1:1000 in 1%BSA/0.2%OvaAlb/PBS 13hours at 4°C.
Secondary antibody: ab205719 - Goat Anti-Mouse IgG H&L (HRP) diluted 1:5000 in TBST.
ECL prime: 4 second exposure.
Predicted size: ~45kDa.

Abcam user community

Verified customer

投稿 Aug 12 2016

Test ab205719 - Goat Anti-Mouse IgG H&L (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Excellent specificity.
Excellent sensitivity.
WB conditions:
- Cell lisates: 20ug protein
- non-reducing
- Primary antibody: Mouse anti-GRP78, clon 40/BiP, Cat. No. 610979. BD Biosciences. Dilution 1:1000
- Secondary antibody: 1:6000
- Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
- Exposure time: 1 second, 3th film out 4

Abcam user community

Verified customer

投稿 Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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