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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (222)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

Key features and details

  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

関連製品

製品の概要

  • 製品名

    Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)
    IgG 二次抗体 製品一覧
  • 由来種

    Goat
  • ターゲット生物種

    Mouse
  • 特異性

    ab150115 is specific to Mouse IgG.

    ab150115 has less than 47% cross-reactivity with rat IgG.

  • アプリケーション

    適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 647

関連製品

  • Alternative Versions

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (ab175473)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175660)
    • Goat Anti-Mouse IgG H&L (ab182017)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (Biotin) (ab207996)
  • Related Products

    • Normal Goat Serum (ab7481)
    • Anti-CD3 antibody [MEM-57] (ab8090)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150115の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (2)
Use at an assay dependent concentration.
ICC/IF (4)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.

ab176103 - Mouse monoclonal IgG1 (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.

特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.

ab176103 - Mouse monoclonal IgG1 (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.

画像

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 647) (ab150115) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) and (ab16048, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 (red) goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab150077 Alexa Fluor® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei.

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    This data was developed using the unconjugated antibody (ab182017).

  • ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

    Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182017).

  • Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
    Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (222)

ab150115 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab150115 は 222 報の論文で使用されています。

  • Lee SG  et al. ?-Tocotrienol-Loaded Liposomes for Radioprotection from Hematopoietic Side Effects Caused by Radiotherapeutic Drugs. J Nucl Med 62:584-590 (2021). PubMed: 32826318
  • Li Y  et al. Songorine promotes cardiac mitochondrial biogenesis via Nrf2 induction during sepsis. Redox Biol 38:101771 (2021). PubMed: 33189984
  • Li C  et al. Changes in the expression of endothelial monocyte-activating polypeptide II in the rat hippocampus following status epilepticus. Int J Mol Med 47:699-707 (2021). PubMed: 33416103
  • Zou W  et al. ASK1/p38-mediated NLRP3 inflammasome signaling pathway contributes to aberrant retinal angiogenesis in diabetic retinopathy. Int J Mol Med 47:732-740 (2021). PubMed: 33416127
  • Yang G & Zhao Y MicroRNA-490-3p inhibits inflammatory responses in LPS-induced acute lung injury of neonatal rats by suppressing the IRAK1/TRAF6 pathway. Exp Ther Med 21:152 (2021). PubMed: 33456519
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

It works well with Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
This Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) works well with Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978) in mouse skin tissue.

Abcam user community

Verified customer

投稿 Sep 17 2020

AlexaFluor647 for Immunofluorescence

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
We used this AlexaFluor647 for IF with different primary antibodies including Gama Tubulin and Acetylated Tubulin for imaging. We used a 1:200 dilution and imaged via LSM 700 confocal microscopy. This dye was used in conjunction with some combination of DAPI, GFP or AlexFluor488(rabbit), and/or AlexaFluor594(rabbit). This dye was excellent, it stained the targets very well and showed little background noise.

REVIEW30

Abcam user community

Verified customer

投稿 Oct 30 2019

An abreview for ab150115 - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
HeLa cells were cultured on cover slips and fixed with 4% paraformaldehyde in PBS (pH7.4) at room temperature for 10 min after exposed upon 10 uM choloroquine for 6 h. After washing three times with PBS, the cells were permeabilized with ice-cold methanol for 2.5 min. After three washes with PBS, the cells were incubated with blocking solution (5% BSA in PBS) for 1 h and then with primary antibody p62 overnight at 4ºC. The next day, the cells were washed five times for 5 min each time with PBS and then incubated with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor 647) for 1 to 2 h. The cells were washed four times with PBS for 5 min each time, and DAPI stained for 15 min. After three washes with PBS, the coverslips were mounted on slides using Fluoro-GEL (Electron Microscopy Sciences). Images were taken using Keyence BZ-X700.

Abcam user community

Verified customer

投稿 Nov 01 2018

Whole-mount immunofluorescence on mouse inner ear sensory epithelia

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Stained fixed sensory epithelia from p15 mice with M-anti-myosin7a overnight in 4 c, and then incubated the tissue for 2 hours in room temp with Abcam's goat anti mouse secondary 647 antibody (ab150115), as well as DAPI.
Staining shows specificity for hair cells.

Mr. Shahar Taiber

Verified customer

投稿 Oct 30 2018

Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam ab150075 - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) in kidney cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vivo studies

DR. Shailendra Singh

Verified customer

投稿 Aug 17 2018

ab150115 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) used with anti-GFP antibody ab38689.

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
Antigen retrieval is optional in this staining. Blocking used was protein block. The left image is abcam's anti-GFP [6AT316] antibody (ab38689) used 1:200, for 12h at RT, with a competitors secondary antibody. On the right, is the same primary antibody, but this time used with abcam's Goat anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115) at 1:1000 dilution, for 3h at RT.

Abcam user community

Verified customer

投稿 May 23 2014

Question

thank you for your mail, I chose ab76055. could you please confirm that the following secondary antibody AB 150115 is suitable for the antibody I'd like to buy?
Thank you in advance for your attention
Kind regards

Read More

Abcam community

Verified customer

Asked on Dec 12 2012

Answer

Thank you for contacting us.

I can confirm, that the Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (ab150115) would be suitable for detection of the Anti-E Cadherin antibody [M168] (ab76055).

The ab150115 is covered by our six month guarantee for ICC/IF, ELISA, IHC-P and Flow Cyt applications.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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