Goat Anti-Human IgG Fc (HRP) (ab97225)
Key features and details
- Goat Anti-Human IgG Fc (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Human IgG Fc (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Human -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
免疫原
Other Immunogen Type corresponding to Human IgG.
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.80
Constituents: 0.2% BSA, 99% PBS, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項（標識）
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97225の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
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| IHC-P |
1/200 - 1/2000.
|
|
| ELISA |
1/10000 - 1/100000. (Primary)
|
|
| WB |
1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000
|
| 特記事項 |
|---|
|
ICC
Use at an assay dependent dilution. |
|
IHC-P
1/200 - 1/2000. |
|
ELISA
1/10000 - 1/100000. (Primary) |
|
WB
1/2000 - 1/25000. Colormetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000 |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab97225 は 21 報の論文で使用されています。
- Demers-Mathieu V et al. Previous viral symptoms and individual mothers influenced the leveled duration of human milk antibodies cross-reactive to S1 and S2 subunits from SARS-CoV-2, HCoV-229E, and HCoV-OC43. J Perinatol 41:952-960 (2021). PubMed: 33649442
- Kim YJ et al. Neutralizing Human Antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 Isolated from a Human Synthetic Fab Phage Display Library. Int J Mol Sci 22:N/A (2021). PubMed: 33671877
- Claro F et al. Immunoglobulin G antibody response to the Sputnik V vaccine: previous SARS-CoV-2 seropositive individuals may need just one vaccine dose. Int J Infect Dis 111:261-266 (2021). PubMed: 34343704
- Stockdale L et al. Cytomegalovirus Antibody Responses Associated With Increased Risk of Tuberculosis Disease in Ugandan Adults. J Infect Dis 221:1127-1134 (2020). PubMed: 31689350
- Thakkar D et al. 10D1F, an Anti-HER3 Antibody that Uniquely Blocks the Receptor Heterodimerization Interface, Potently Inhibits Tumor Growth Across a Broad Panel of Tumor Models. Mol Cancer Ther 19:490-501 (2020). PubMed: 31911530