Anti-Glutathione Peroxidase 4 抗体 (ab40993)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glutathione Peroxidase 4 antibody
Glutathione Peroxidase 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glutathione Peroxidase 4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: a wide range of other species -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Glutathione Peroxidase 4.
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ポジティブ・コントロール
- ab40993 gave a positive signal in the following human tissue lysates: Brain, Testis, Liver, Heart, Skeletal Muscle, Kidney, Colon, Small Inestine (data not shown).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
組織特異性
Present primarily in testis. -
配列類似性
Belongs to the glutathione peroxidase family. -
細胞内局在
Mitochondrion. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2879 Human
- Omim: 138322 Human
- SwissProt: P36969 Human
- Unigene: 433951 Human
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別名
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
画像
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All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lane 7 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab40993 は 7 報の論文で使用されています。
- Li W et al. Lipopolysaccharide-induced splenic ferroptosis in goslings was alleviated by polysaccharide of atractylodes macrocephala koidz associated with proinflammatory factors. Poult Sci 101:101725 (2022). PubMed: 35299067
- Zhou X et al. Polysaccharide of Atractylodes macrocephala Koidz Alleviates Cyclophosphamide-Induced Thymus Ferroptosis in Gosling. Animals (Basel) 12:N/A (2022). PubMed: 36496913
- Sheng S et al. Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC. Oxid Med Cell Longev 2021:6241242 (2021). PubMed: 34760046
- Li R et al. Transcriptome Investigation and In Vitro Verification of Curcumin-Induced HO-1 as a Feature of Ferroptosis in Breast Cancer Cells. Oxid Med Cell Longev 2020:3469840 (2020). PubMed: 33294119
- O'Brien KM et al. The loss of hemoglobin and myoglobin does not minimize oxidative stress in Antarctic icefishes. J Exp Biol 221:N/A (2018). PubMed: 29361578
- Hauser DN et al. Dopamine quinone modifies and decreases the abundance of the mitochondrial selenoprotein glutathione peroxidase 4. Free Radic Biol Med 65:419-27 (2013). PubMed: 23816523
- Li W et al. Repression of endometrial tumor growth by targeting SREBP1 and lipogenesis. Cell Cycle 11:2348-58 (2012). PubMed: 22672904