Application
Western blot
Sample
Mouse Cell lysate - whole cell (BMDM)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris gel, MES running buffer)
Loading amount
5 µg
Specification
BMDM
Blocking step
Licor TBS blocking buffer as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/3000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
IR
Exposure
1 second(s)
Bands
Specific: 30 kDa
Positive control
left lane: human Gal3 positive control
Additional data
Additional Notes
lane 1: markers
lanes 2-6: primary ms BMDM with different treatments
lane 7: human Gal3 positive control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 24 2016