Application
Western blot
Sample
Human Cell lysate - whole cell (Mesothelial cell line MeT-5A (ATCC CRL-9444))
Loading amount
4 µg
Specification
Mesothelial cell line MeT-5A (ATCC CRL-9444)
Gel Running Conditions
Reduced Denaturing (GE Healthcare, Excelgel 12.5 homogenous)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST (1%w/v non-fat dry milk)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 24 kDa
Additional data
PubMed ID
19231869: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 11 2009