Anti-GRB2 抗体 [Y237] (ab32037) | アブカム

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製品名

Anti-GRB2 antibody [Y237]
GRB2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [Y237] to GRB2
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
ポジティブ･コントロール
- WB: HEK293, PC-12 and NIH/3T3 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
特記事項

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

Y237
アイソタイプ

IgG
研究分野

Alternative Versions

Our Abpromise guarantee covers the use of ab32037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
Flow Cyt		1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP		1/50.
IHC-P		1/100 - 1/250.
ICC/IF		1/50 - 1/100.

機能

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
配列類似性

Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
ドメイン

The SH3 domains mediate interaction with RALGPS1 and SHB.
細胞内局在

Golgi apparatus.
Target information above from: UniProt accession P62993 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
- SwissProt: P62994 Rat
- Unigene: 444356 Human
- Unigene: 708119 Human
- Unigene: 439649 Mouse
- Unigene: 3360 Rat
see all
別名
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
- Ash protein antibody
- EGFRBP GRB2 antibody
- Epidermal growth factor receptor binding protein antibody
- Epidermal growth factor receptor binding protein GRB2 antibody
- GRB 2 antibody
- GRB2 adapter protein antibody
- Grb2 antibody
- GRB2_HUMAN antibody
- Grb3 3 antibody
- Growth factor receptor bound protein 2 antibody
- Growth factor receptor bound protein 3 antibody
- Growth factor receptor-bound protein 2 antibody
- HT027 antibody
- MST084 antibody
- MSTP084 antibody
- NCKAP2 antibody
- OTTHUMP00000166096 antibody
- OTTHUMP00000166097 antibody
- OTTHUMP00000166098 antibody
- Protein ash antibody
- SEM5 antibody
- SH2/SH3 adapter GRB2 antibody
see all

Western blot - Anti-GRB2 antibody [Y237] (ab32037)

All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) cell lysate
Lane 3 : HEK293 (human epithelial cell line from embryonic kidney) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking/Dilution buffer : 5% NFDM/TBST

Exposure time : 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)

Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)

Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037.
Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)

Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GRB2 (red) with ab32037 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-GRB2 antibody [Y237] (ab32037)This image is a courtesy of Anonymous Abreview

Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 25 kDa
Observed band size: 24 kDa (why is the actual band size different from the predicted?)

Exposure time: 1 minute
See Abreview
Western blot - Anti-GRB2 antibody [Y237] (ab32037)

Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Recombinant human AHA1 protein (ab80352) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Exposure time: 2 minutes

This product has been referenced in:

Arbulo-Echevarria MM et al. A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling. Front Immunol 9:115 (2018). Read more (PubMed: 29456532) »
Majumder P et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). IHC . Read more (PubMed: 28360125) »

See all 7 Publications for this product

ab32037 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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レビュー
Q&A

レビューを送る質問を送る

3 reviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (D3)

Permeabilization

Yes - 0.1% Triton

Specification

D3

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 1%

Fixative

Paraformaldehyde

abreview image

Abcam user community

Verified customer

投稿 Oct 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (Mesothelial cell line MeT-5A (ATCC CRL-9444))

Loading amount

4 µg

Specification

Mesothelial cell line MeT-5A (ATCC CRL-9444)

Gel Running Conditions

Reduced Denaturing (GE Healthcare, Excelgel 12.5 homogenous)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

abreview image

Abcam user community

Verified customer

投稿 Sep 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (Jurkat)

Loading amount

500000 cells

Specification

Jurkat

Gel Running Conditions

Reduced Denaturing

Blocking step

Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

abreview image

Abcam user community

Verified customer

投稿 Dec 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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