Application
Immunocytochemistry
Sample
Rat Cultured Cells (primary neuronal culture_Hippocampal neuron, DIV16)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
primary neuronal culture_Hippocampal neuron, DIV16
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 3% BSA, 0.1% Tween 20 in PBS
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
We would like to thank our customer for submitting this valuable feedback in form of an AbReview. Our laboratory has tested this antibody before and found that this antibody failed with rat samples in our hands, therefore, we are unable to guarantee for the use of this antibody in Rat samples, unfortunately, although it worked well for this customer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Junmo Hwang
Verified customer
投稿 Apr 27 2016