Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-15% SDS-PAGE)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 130 kDa Non-specific: 250 (dimer) kDa
Exposure
45 second(s)
Additional data
Additional Notes
Line 1 - reducing agent 1% 2-Mercaptoethanol
Line 2 - reducing agent 5% 2-Mercaptoethanol
This Ab is appropriate for detection of GM130 dimer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
投稿 Nov 19 2014