The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
Gcm2, a mouse ortholog of the Drosophila Glial Cells Missing gene, is expressed in the parathyroid-specific domains in the 3rd pouches from E9.5. The null mutation of Gcm2 causes aparathyroidism in the fetal and adult mouse and has been proposed to be a master regulator for parathyroid development. During Drosophila embryogenesis Gcm2 plays a crucial role in promoting glial cell differentiation.
All lanes : Anti-GCM2 antibody (ab64723) at 1 µg/ml
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate Lane 2 : Brain (Mouse) Tissue, 0 days old Lane 3 : Skeletal Muscle (Mouse) Tissue Lane 4 : Thymus (Rat) Tissue Lane 5 : Thyroid (Mouse) Tissue
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa Observed band size: 56 kDa Additional bands at: 37 kDa, 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.