Anti-GAG 抗体 (ab100970)
Key features and details
- Rabbit polyclonal to GAG
- Suitable for: WB, ELISA
- Reacts with: Murine leukemia virus
- Isotype: IgG
製品の概要
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製品名
Anti-GAG antibody -
製品の詳細
Rabbit polyclonal to GAG -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Murine leukemia virus -
免疫原
Recombinant Murine Leukemia Virus GAG p30 capsid protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100970の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1500 - 1/2000. Predicted molecular weight: 30 kDa.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1500 - 1/2000. Predicted molecular weight: 30 kDa. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Murine leukemia viruses belong to family of Retroviruses group and are named for their ability to cause cancer in mice. Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important roles at several stages in the viral life cycle. Late in infection, the Gag precursor protein acts to mediate the assembly and release of virion particles. After budding, the core matures by cleavage of Gag precursor protein into the mature core proteins, the matrix (MA), capsid (CA) and nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of infection.
画像
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All lanes : Anti-GAG antibody (ab100970) at 1/1500 dilution
Lane 1 : PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas);
Lane 2 : HEK293 cells
Lane 3 : GAG protein expressed in HEK293 cells
Lane 4 : PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas);
Lane 5 : CHO cells
Lane 6 : Gag protein expressed in CHO cells
Secondary
All lanes : Alkaline phosphatase conjugated anti rabbit IgG at 1/5000 dilution
Predicted band size: 30 kDa
データシートおよび資料
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Datasheet download
参考文献 (6)
ab100970 は 6 報の論文で使用されています。
- Uygur B et al. Syncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells. Sci Rep 9:17637 (2019). PubMed: 31776415
- Borrenberghs D et al. Post-mitotic BET-induced reshaping of integrase quaternary structure supports wild-type MLV integration. Nucleic Acids Res N/A:N/A (2018). PubMed: 30445610
- Zhou B et al. miR-27 inhibits the NF-?B signaling pathway by targeting leptin in osteoarthritic chondrocytes. Int J Mol Med 40:523-530 (2017). PubMed: 28627582
- Stabell AC et al. Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins. PLoS Pathog 12:e1006066 (2016). WB . PubMed: 28027315
- McNally DJ et al. Optimised concentration and purification of retroviruses using membrane chromatography. J Chromatogr A 1340:24-32 (2014). WB ; Mouse . PubMed: 24685165
- Yin D et al. Convection-enhanced delivery improves distribution and efficacy of tumor-selective retroviral replicating vectors in a rodent brain tumor model. Cancer Gene Ther 20:336-41 (2013). PubMed: 23703472