Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% BSA + 0.3% Triton X
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Abcam response
Abtrial: untested species, guinea pig
IHC-P
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Poonam Rani
Verified customer
投稿 Apr 07 2016