Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Immuno-precipitation step
Protein A
Other product details
Concentration
1.5 µg/ml
Incubation time
4 hour(s) and 0 minute(s)
Western Blot antibody
Name
Non-Abcam antibody was used: Amersham
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This Ab works fine for a Western Blot but does not work well for IP. I could not resolve a specific band for GbL although I was counterstaining with the same Ab resulting in very high background. I could also not resolve mTOR or Raptor using Santa Cruz goat derived Ab on a Western after attempting the GbL IP. IP of mTOR with the Santa Cruz N-19 and subsequent blot with this GbL Ab will show specific band at 35 Kda
Abcam response
Thank you for sharing your results with us. This product has not previously been tested for application in IP and it seems that ab25974 is unsuitable for this assay. We believe that our customers will find this information very useful.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Stephen Dann
Verified customer
投稿 Dec 20 2005