Anti-FOXK1/MNF 抗体 (ab85999)
Key features and details
- Rabbit polyclonal to FOXK1/MNF
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXK1/MNF antibody
FOXK1/MNF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXK1/MNF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human FOXK1/MNF aa 683-733.
Database link: NP_001032242.1 -
ポジティブ・コントロール
- Whole cell lysate from HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab85999 was affinity purified using an epitope specific to FOXK1 / MNF immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 75 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. -
組織特異性
Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node. -
配列類似性
Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 221937 Human
- Entrez Gene: 17425 Mouse
- SwissProt: P85037 Human
- SwissProt: P42128 Mouse
- Unigene: 487393 Human
- Unigene: 24214 Mouse
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別名
- A630048H08Rik antibody
- AI463295 antibody
- ENSMUSG00000075577 antibody
see all
画像
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All lanes : Anti-FOXK1/MNF antibody (ab85999) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FOXK1/MNF with ab85999 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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Detection of FOXK1/MNF by Western blot of Immunprecipitate.
ab85999, at 1 µg/ml, staining FOXK1 / MNF in HeLa whole cell lysate immunoprecipitated using ab85999 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-FOXK1/MNF antibody (ab85999) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Control IgG
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab85999 は論文での使用が確認できていません。