Anti-FLI1 抗体 (ab90583)
Key features and details
- Rabbit polyclonal to FLI1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FLI1 antibody
FLI1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FLI1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Chicken, Cow, Human -
免疫原
Synthetic peptide corresponding to Human FLI1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101301) -
ポジティブ・コントロール
- This antibody gave a positive signal in MEF1 and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab90583の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
関連疾患
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Entrez Gene: 315532 Rat
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
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別名
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
画像
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All lanes : Anti-FLI1 antibody (ab90583) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa, 31 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab90583 は論文での使用が確認できていません。