製品の概要

  • 製品名
    FITC Conjugation Kit
    FITC キット 製品一覧
  • 製品の概要

    FITC Conjugation Kit ab102884 uses a simple and quick process to conjugate an antibody to FITC . It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to FITC using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to FITC.

  • 特記事項

    Amount and volume of antibody for conjugation to FITC 

     Kit size   Recommended 
    amount of antibody
    1 
    Maximum 
    amount of antibody
    Maximum antibody 
    volume2
    30 µg   3 x 10 µg  3 x 20 µg 3 x 10 µL
    300 µg   3 x 100 µg  3 x 200 µg 3 x 100 µL
    1 mg   1 mg 2 mg 1 mL

    1 Using the maximum amount of antibody may result in less labelling per antibody.

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.


    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture 

  • アプリケーション
    適用あり: Conjugationmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Conjugation Use at an assay dependent dilution.

画像

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

プロトコール

参考文献

This product has been referenced in:
  • Weagel EG  et al. Biomarker analysis and clinical relevance of TK1 on the cell membrane of Burkitt's lymphoma and acute lymphoblastic leukemia. Onco Targets Ther 10:4355-4367 (2017). Read more (PubMed: 28919785) »
  • Apostolou P  et al. Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection. J Biol Res (Thessalon) 24:11 (2017). Read more (PubMed: 28975085) »

See all 8 Publications for this product

レビューと Q&A

FITC Conjugated full length EpCAM Protein

Excellent Excellent 5/5 (Ease of Use)
Abreviews
FITC was succesfully Conjugated with Human EpCAM Protein. It was quite useful.
Username

Abcam user community

Verified customer

投稿 Nov 02 2015

There is no difference in sensitivity between the rapid (eg. ab188285) and the standard (eg. ab102884) labeling kits. The only difference between the two is the incubation time required for the reaction.

Better results than with secondary antibody.

Excellent Average 3/5 (Ease of Use)
Abreviews
The kit was easy enough for me to hand this off to an undergraduate, albeit with some explanation of the directions. These were a tad bit confusing at first, and not really clear on the volume of antibody to add (they give you a couple options since the Ab concentration of course matters, but didn't make it clear they were different options).
Otherwise the kit work fantastically with some store bought, pure, concentrated antibody! It actually gave better results than our previous method of using a conjugated secondary antibody when used in Flow Cytometry after doing an Intracellular Stain.
Username

Hans Wilms

Verified customer

投稿 Jun 03 2014


This antibody will detect the whole cells, and because the antiserum has not been absorbed (only Protein A purified) it may react with related microorganisms.


As this antibody is suitable for conjugation purpose according to the data...

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Thank you for your enquiry.

The EasyLink conjugation kits are designed so that they can be used to label proteins other than antibodies. In this case you should be able to label the Fc fused protein in the same way as labeling an antibody. H...

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Thank you for contacting us.

The labeling kit works by targeting free amines groups on your antibody. We therefore cannot guarantee that the label will not bind to the FAB area, however this would be the case for any other amine orientated lab...

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Thank you for your inquiry and your interest in our products.

I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received th...

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Thank you for your interest in the FITC conjugation kit. Unfortunately, it will be difficult to achieve a consistent ratio of FITC : IgG without extensive testing of the kit to determine the appropriate conditions. We have not done this testing, as the...

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Thank you for contacting us.

The fluorescein in ourKit is not simply fluorescein and it is not FITC either. Our fluorescein has been specially treated with proprietary Easy-Link chemistry to make the unique one-step conjugation possible.
<...

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Thank you for your inquiries.

I can confirm that additives in the buffer of the antibody will interfere with the labeling reaction.

We offer a kit that will concentrate the antibodyand also remove the additives:

ab102776 <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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